The Egyptian Society of Biochemistry and Molecular BiologyThe Egyptian Journal of Biochemistry and Molecular Biology1687-150236120181201Association of Protein Z intronic G79A Polymorphism with susceptibility to deep vein thrombosis in Egyptian patients with Behcet's disease: A preliminary report1161984910.21608/ejb.2018.19849ENRashaGaberMedical Biochemistry, Faculty of Medicine, Tanta University, EGYPTHanaaGaballahMedical Biochemistry, Faculty of Medicine, Tanta University, EGYPTSamahElmedanyRheumatology Departments , Faculty of Medicine, Tanta University, EGYPTJournal Article20170918Behçet disease (BD) is a multi-systemic inflammatory disorder, which can affect all types of blood vessels. Thrombotic vasculopathy is one of the major causes complicating the clinical course of BD. This study aims to investigate potential associations between G79A polymorphisms of the protein Z (PZ) gene and venous thrombosis as well as other clinical manifestations in Egyptian patients with BD. Sixty patients who satisfied the International Study Group criteria for BD and sixty healthy age and sex- matched control subjects were genotyped for G79A polymorphisms of the PZ gene by restriction fragment length polymorphism PCR. Plasma levels of PZ were estimated using enzyme linked immunosorbent assay kit. Our preliminary data revealed that, compared to the GG genotype, the AA and AG PZ intron F genotypes were significantly associated with BD susceptibility and with reduced plasma PZ levels. The patients were then subgrouped according to Protein Z G79A genotypes to examine the G79A genotype frequencies according to the clinical characteristics, where there was a statistically significant association between AG and AA genotypes frequencies and deep venous thrombosis. In conclusion, our study nominates the minor A allele of the PZ G79A polymorphism as a discriminatory genetic marker for identification of BD patients at high risk for developing thrombotic events. However, large scaled studies are needed to verify these preliminary results.https://ejb.journals.ekb.eg/article_19849_c59eefbc5a8559dac7aa5e2efda366e4.pdfThe Egyptian Society of Biochemistry and Molecular BiologyThe Egyptian Journal of Biochemistry and Molecular Biology1687-150236120181201PLASMA LEVELS OF CHEMERIN, LEPTIN AND PSORIASIN AS POTENTIAL MARKERS OF SUBCLINICAL ATHEROSCLEROSIS IN PSORIASIS PATIENTS17341985010.21608/ejb.2018.19850ENNeveinAl-sheikhMedical Biochemistry& Molecular Biology, Faculty of Medicine, Menoufia University, EgyptWafaaShehataDermatology & Andrology, Faculty of Medicine, Menoufia University, EgyptShaimaaHassaneinDiagnostic Radiology, Faculty of Medicine, Menoufia University, EgyptWaleedIbrahimCardiology Departments, Faculty of Medicine, Menoufia University, Egypt.Journal Article20180401Chemerin, leptin and psoriasin are pro-inflammatory and immune-modulatory proteins associated with psoriasis and displayed higher circulating levels. Their relation to atherosclerosis in psoriatic patients has been investigated in numerous studies with wide-ranging results. Therefore, the present study aimed to assess plasma levels of chemerin, leptin and psoriasin and evaluate their relationship with carotid intima-media thickness (CIMT) and epicardial fat thickness (EFT) as potential predictors for subclinical atherosclerosis in psoriasis patients. The study included fifty psoriatic patients and forty age and gender matched healthy controls. Clinical severity of psoriasis was evaluated by Psoriasis Area and Severity Index (PASI). Fasting blood glucose and lipid profile were estimated. Plasma levels of high sensitivity-CRP (hs-CRP), chemerin, leptin and psoriasin were measured by ELISA. CIMT and EFT were assessed by Ultrasonography and Echocardiography, respectively. Plasma levels of hs-CRP, chemerin, leptin and psoriasin as well as CIMT and EFT were significantly elevated in psoriasis patients compared to controls. CIMT and EFT were significantly positively correlated with PASI, plasma hs-CRP, chemerin, leptin and psoriasin. Moreover, significant positive correlation was demonstrated between PASI and plasma hs-CRP, chemerin, leptin and psoriasin. Multiple linear regression analyses showed that chemerin, leptin and psoriasin were
independently correlated with CIMT and EFT and exhibited high<br />significance for predicting their values. It can be concluded that<br />chemerin, leptin and psoriasin might represent an important link<br />between psoriasis and atherosclerosis. Measurements of plasma<br />chemerin, leptin and psoriasin along with CIMT and EFT seem to be<br />valuable potential markers of subclinical atherosclerosis in patients<br />with psoriasis.https://ejb.journals.ekb.eg/article_19850_5fe783070c1ae1d3cb04d0ecd495b40b.pdfThe Egyptian Society of Biochemistry and Molecular BiologyThe Egyptian Journal of Biochemistry and Molecular Biology1687-150236120181201ASSOCIATION OF NEUROPEPTIDE-Y -399 (T/C) GENE POLYMORPHISM AND ITS LEVEL WITH THE RISK OF TYPE-2 DIABETES MELLITUS49671985110.21608/ejb.2018.19851ENYasserElghobashyMedical Biochemistry,Faculty of Medicine, Menoufia University, EgyptEmanAbd El GayedMedical Biochemistry, Faculty of Medicine, Menoufia University, EgyptShimaaZewinInternal Medicine Departments
Faculty of Medicine, Menoufia University, EgyptJournal Article20180422Neuropeptide-Y (NPY) is widely distributed centrally and peripherally and plays an important role in regulating glucose metabolism, energy metabolism and vascular function. Several studies had found that NPY is involved in the pathophysiology of type-2 diabetes Mellitus (T2DM). The aim of this study was to evaluate the association of NPY -399 (T/C) gene polymorphism and its serum level with susceptibility to T2DM. Seventy diabetic patients and seventy age and gender matched healthy controls were enrolled in this study. Serum NPY was measured by ELISA and NPY rs16147(-399 T/C) polymorphism was analyzed using the TaqMan allelic discrimination assay technique. Results showed significant statistical differences between the two studied groups regarding serum NPY level and NPY rs16147 (-399T/C) genotype distribution with increased serum NPY level and increased frequency of the CC and TC genotype in patients with diabetes mellitus. Conclusion: CC genotypes of NPY (-399 T/C) gene polymorphism and its associated high serum NPY level might be a genetic risk factor for T2DM.https://ejb.journals.ekb.eg/article_19851_ea79793bc5f916d540aebc4fc1825613.pdfThe Egyptian Society of Biochemistry and Molecular BiologyThe Egyptian Journal of Biochemistry and Molecular Biology1687-150236120181201LNC RNA HULC AS A NOVEL DIAGNOSTIC AND THERAPEUTIC TARGET IN PREECLAMPSIA65781985210.21608/ejb.2018.19852ENOmaymaAbdelaleemDepartment of Medical Biochemistry and Molecular Biology, Faculty of Medicine, Fayoum UniversityRaniaMahmoudDepartment of Medical Biochemistry and Molecular Biology, Faculty of Medicine, Fayoum UniversityOlfatShakerDepartment of Medical Biochemistry and Molecular Biology, Faculty of Medicine, Cairo University.NadaHemedaDepartment of Genetics, Faculty of Agriculture, Fayoum UniversityNaglaaAhmedDepartment of physiology, Faculty of Medicine, Zagazig UniversityJournal Article20181125Background: Preeclampsia is a severe pregnancy-related disease mainly in developing countries. Non-coding RNAs are very important factors in regulating the expression of certain genes and are involved in pathogenesis of numerous diseases. Recently, non coding RNA biomarkers are used as diagnostic tools for different disorders such as PE.<br />Methods: lnc RNA HULC expression levels was measured in serum collected from 22 patients with PE and 23 healthy pregnancies, using real-time PCR.<br />Results: HULC expression was elevated reaching significant levels (p < 0.001with 2.16 fold change) in patients compared with control group. HULC was markedly increased in both severe and mild preeclamptic subgroups when compared to healthy controls (p<0.001 with 1.97 fold change and p<0.001 with 2.16 fold change respectively). ROC curve was assessed, the AUC of lnc RNA HULC was 0.952 (95%, CI=0.904 -1.000, P< 0.001).<br />Conclusion: lnc RNA HULC may be involved in PE pathogenesis and may be used as potential non-invasive diagnostic biomarker for this disease.https://ejb.journals.ekb.eg/article_19852_ef49977b5fc3d73e2623389525ed2110.pdfThe Egyptian Society of Biochemistry and Molecular BiologyThe Egyptian Journal of Biochemistry and Molecular Biology1687-150236120181201A PILOT STUDY SCOUTING THE ASSOCIATION OF HOTAIR GENETIC POLYMORPHISMS WITH GASTRIC CANCER RISK IN SOME EGYPTIAN PATIENTS1011151985310.21608/ejb.2018.19853ENAzzaElamirMedical Biochemistry, Faculty of
Medicine, Fayoum University, Fayoum, Egypt.HassanEl SayedMedical Biochemistry, Faculty of
Medicine, Fayoum University, Fayoum, Egypt.MohamedIbrahimGeneral Surgery Departments, Faculty of
Medicine, Fayoum University, Fayoum, Egypt.Journal Article20181125Gastric cancer is one of the prevalent malignant tumors all over the<br />world. In the current study we aimed at elucidating a possible correlation<br />between LncRNA-HOTAIR polymorphisms rs7958904 G>C and<br />rs874945 G>A and risk of developing gastric cancer in Egyptian patients.<br />Blood samples were collected from controls and patients. We performed<br />real-time polymerase chain reaction (RT-PCR) for genotyping of the two<br />variant alleles at the SNPs sites of HOTAIR gene in all subjects. As regard<br />rs7958904 SNP, there was statistically significant higher mutant genotype<br />CC and Mutant C allele among gastric cancer patients and as regard<br />rs874945 SNP, there was statistically significant higher mutant genotype<br />AA and Mutant A allele among gastric cancer patients. As regard<br />rs874945 SNP, the mutant genotype AA and the Mutant A allele were<br />statistically significantly higher in Positive H. pylori patients than negative<br />H. pylori patients. In Conclusion, the mutant genotypes (CC and AA) of<br />rs7958904and rs874945 SNPs of LncRNA-HOTAIR gene predominate in<br />gastric cancer patients that would displayed their impact in increasing the<br />risk of developing gastric cancer in Egyptian patients, while the wild<br />genotypes (GG and GG) predominate in controls which support our<br />hypothesis.https://ejb.journals.ekb.eg/article_19853_bb9935c9617db0b68ca2b5a2f5d351eb.pdfThe Egyptian Society of Biochemistry and Molecular BiologyThe Egyptian Journal of Biochemistry and Molecular Biology1687-150236120181201The study of mRNA Expression of Dopamine Receptors D2 and D3 in Schizophrenic Patients791001986710.21608/ejb.2018.19867ENAzzaAbdu AllahMedical Biochemistry and Molecular Biology Department, Faculty of Medicine, Menoufia University, EgyptEmanAbd El GayedMedical Biochemistry and Molecular Biology Department Faculty of Medicine, Menoufia University, EgyptAmrShalabyPsychiatry Department
, Faculty of Medicine, Menoufia University, EgyptJournal Article20181126Schizophrenia is a severe mental disorder affecting behavior, thought and emotions in the form of hallucinations, delusions and cognitive impairment. Dopamine system is implicated in the pathogenesis of schizophrenia through dopamine receptors expression in the brain. The aim of this work is to evaluate the role of mRNA expression of dopamine receptors D2 and D3 in the diagnosis of schizophrenia. Thirty-five schizophrenic patients and fifteen age and gender matched healthy controls were included in this study. Serum lipid profiles were determined by enzymatic colorimetric method while the determination of dopamine receptors D2 and D3 mRNA expression was performed by real time q PCR technique. There was significant statistical decrease of HDL-c and increase of LDL-c in patients compared to control group, while Cholesterol and TAG showed no significant statistical difference between the two groups. There was significant increase of the mRNA expression of dopamine receptors D2 and D3 genes in the blood of the patients compared to control group (p value= 0.001 and < 0.001 respectively). From this study, it could be concluded that Dopamine receptors D2 and D3 mRNA expression in the blood might be used as diagnostic markers of schizophrenia, revealing the importance of dopamine receptors antagonist as antipsychotics.https://ejb.journals.ekb.eg/article_19867_31dfb0f73147e9b600e01b2a4de37a0c.pdfThe Egyptian Society of Biochemistry and Molecular BiologyThe Egyptian Journal of Biochemistry and Molecular Biology1687-150236120181201Insulin-like Growth Factor 2 Binding Protein 2 Gene Polymorphism in Egyptian Patients with Type 2 Diabetes35481987310.21608/ejb.2018.19873ENMohammedHajarMedical Biochemistry and Molecular Biology, Faculty of Medicine, Suez Canal University, Round
Road, Ismailia, EgyptNagwanSabekMedical Biochemistry and Molecular Biology, Faculty of Medicine, Suez Canal University, Round
Road, Ismailia, EgyptAmanyEl-kazazMedical Biochemistry and Molecular Biology, Faculty of Medicine, Suez Canal University, Round
Road, Ismailia, EgyptFathallaHassanMedical Biochemistry and Molecular Biology Faculty of Medicine, Suez Canal University, Round
Road, Ismailia, EgyptAbdelraoufEldeibInternal Medicine
Departments, Faculty of Medicine, Suez Canal University, Round
Road, Ismailia, EgyptJournal Article20181126Genome-wide association studies (GWAS) identified novel genes associated with type 2 diabetes mellitus (T2DM) which have been replicated in different ethnic populations and yielded inconsistent results. The insulin-like growth factor mRNA-2 binding protein 2 (IGF2BP2) is highly expressed in pancreatic islets, which play roles in normal embryonic growth and development. Our study aimed to replicate the association between insulin growth factor 2 mRNA binding protein 2 (IGF2BP2) gene (rs4402960) variant and T2DM in Egyptian diabetic patients resident in Ismailia city. The study included 152 subjects (76 unrelated T2DM patients and 76 control subjects) who were genotyped by polymerase chain reaction-restriction fragment length polymorphism technique (PCR- RFLP). Age, sex, blood pressure, BMI and Waist Circumference were recorded, and blood glucose, serum triglyceride, total cholesterol (TC), low-density lipoprotein-cholesterol (LDL-C), high-density lipoprotein-cholesterol (HDL-C) and homeostatic model assessment (HOMA) indices were determined. Fasting serum insulin and IGF2BP2 protein levels were analyzed by ELISA. For rs440960 variant of IGF2BP2 gene, risk allele T frequency was associated with T2DM [OR (95% CI)=1.82 (1.14- 2.93), P= 0.012]. The frequency of T/T genotype versus (GG+G/T) genotypes was significantly higher in T2DM patients compared to controls (22.4% vs. 77.6% and 5.3% vs. 94.7%, respectively), (P=0.002). This association remained significant under additive (P=0.003) and co-dominant (P=0.009) genetic models. From this study, it could be concluded that IGF2BP2 rs4402960 polymorphism was found to be significantly associated with the increased risk of T2DM.https://ejb.journals.ekb.eg/article_19873_b3e36b646fdd991f9dd8f789a8032ace.pdf