POSSIBLE MECHANISM OF DISINTEGRIN/LIKE DOMAIN IN MESENCHYMAL STEM CELLS HOMING IN MICE LIVER INJURY MODEL

Document Type : Original Article

Authors

DEPARTEMENT OF MEDICAL BIOCHEMISTRY AND MOLECULAR BIOLOGY, FACULTY OF MEDICINE, AIN SHAMS UNIVERSITY, CAIRO, EGYPT.

Abstract

Liver disease is one of the major health problems in many
countries. Mesenchymal Stem cells have opened a new approach to
deal with liver fibrosis. Improvement of bone marrow derived
mesenchymal stem cellshoming after systemic injection of
disintegrin/like domain purified from Egyptian horned Cerastes
cerastes crude venomwhichwas shown byprevious study. This work
was designed to investigate the possible mechanism of disintegrin/like
domainin mesenchymal stem cells homing to fibrotic liver of CCl4
treated mice. Quantitative gene expression was performed for stromal
cell-derived factor-1 to study homing of bone marrow derived
mesenchymal stem cells, transforming growth factor beta 1 to study
bone marrow derived mesenchymal stem cellshoming and
regeneration of liver cells and hepatocyte growth factor to study
regeneration of liver cells in CCl4 induced liver injury in white albino
mice. It was found that disintegrin/like domaincould increase homing
ofbone marrow derived mesenchymal stem cells as showed by the
significant increase in liver stromal cell-derived factor -1 gene
expression, transforming growth factor beta 1 as well as hepatocyte
growth factor genes expression compared to the non disintegrin/like
domaintreated CCl4 group.

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